Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)

Table 3 Stability values of candidate reference genes as calculated by NormFinder in datasets A-J¹.

¹Datasets consist of (A) all 442 perennial ryegrass tissue samples, (B) 422 field-grown samples harvested following different defoliation management, (C) 13 laboratory-grown samples, (D) 218 perennial ryegrass stubble samples, (E) 220 perennial ryegrass leaf samples, (F) four perennial ryegrass callus, inflorescence and root samples, (G) five perennial ryegrass etiolated seedlings of different cultivars, (H) four field-grown samples harvested at the peak of each season, (I) three laboratory-grown samples to evaluate water stress and (J) four laboratory-grown samples to evaluate cold stress.
²NormFinder analysis carried out using the group property to identify the six different defoliation treatments contained within this dataset. As well as identifying the best reference gene; this analysis gives the combination of the two best reference genes with their combined stability value.

ISSN: 1471-2199