Table 5 qPCR parameters for candidate reference genes
Gene | Forward primer (conc.) | Reverse primer (conc.) | Annealling Temp. (°C) | Amplicon Size (bp) | Amplification efficiency | |||
|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Â | Liver | Ovary | ||
| Â | Â | Â | Â | Â | 2008 | 2009 | 2008 | 2009 |
eef1 | cccctgcaggatgtctacaa (200 nM) | agggactcatggtgcatttc (200 nM) | 64 | 152 | 98% | 95% | 94% | 95% |
Actb | aatcctgcggtatccatgag (250 nM) | gccagggatgtgatctcttt (250 nM) | 62 | 154 | 101% | 105% | 104% | 102% |
odc1 | ggacgactcaaaggcagtgt (500 nM) | ccaatgtccagaagggtcat (500 nM) | 64 | 234 | 96% | 104% | 96% | 104% |
18s | ggatgcgtgcatttatcaga (200 nM) | cgaaagttgatagggcagaca (200 nM) | 64 | 145 | 103% | 105% | 101% | 101% |
l36 | cctgaccaagcagaccaagt (250 nM) | tctctttgcacggatgtgag (250 nM) | 62 | 160 | 101% | 102% | 102% | 101% |
nop14 | gagagcgagagaggctgaag (250 nM) | tttccactctccctcctgtg (250 nM) | 62 | 185 | 101% | 101% | 100% | 100% |