Figure 2

Intergroup transcript abundance variability of each candidate reference genes in liver (A) and ovary (B). Data are based on the threshold cycle (Ct) values of qPCR of reference gene candidates on liver and ovary samples taken in summer 2009. Box-and-whisker plots denote median, upper and lower quartiles, and 10^th and 90^th percentiles of data. Dashed lines within bars denote the means. A suitable reference gene should have Ct values that are similar across the three groups (e.g., l36 in ovary).