Table 1 Oligonucleotide primers used to amplify the E. sinensis FABP gene
Primer name | Sequence (5'-3') | Application |
|---|---|---|
T7 | TAATACGACTCACTATAGG | cDNA cloning |
SP6 | ATTTAGGTGACACTATAGAA | cDNA cloning |
5'SP1 | CCCACAGCACTGAGCCCAATC | Genome walking |
5'SP2 | TATTTCCCGGTGATGGACATGA | Genome walking |
5'SP3 | GGTAGAAACAGCGAGGGCAACT | Genome walking |
3'SP1 | CACCTCCGCCAAGATTGTTATT | Genome walking |
3'SP2 | CGCACTTGAAAGAACCATAGCAG | Genome walking |
3'SP3 | GCTTTATGAGATTCGTTTGCGTGAT | Genome walking |
F-R2 | CAGAAGATGTTACAAGACTAAAG | RT-PCR |
F-S2 | GAGTTGCCCTCGCTGTTTGCTAT | RT-PCR |
F-R3 | CGTGGTCTTGATGACGATGT | RealTime-PCR |
F-S3 | TGGCTCAGTGCTGTGGGGT | RealTime-PCR |
Actin-R | CTCCTGCTTGCTGATCCACATC | RT & RealTime-PCR |
Actin-S | GCATCCACGAGACCACTTACA | RT & RealTime-PCR |