Figure 4
From: High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library
Molecular characterization of the medaka atrS1339X mutant. (A) Sequence alignment of vertebrate ATR (Hs, Homo sapiens; Mm, Mus musculus; Tr, Takifugu rubripes; Ol, Oryzias latipes) in exon 24. Conserved residues are highlighted by white letters on a black background. Red letter and arrow indicate the mutation site. (B) Sequence traces of the region of the the point mutation (arrows) in the founder fish. (C) Quantitative PCR showed a decrease to 70% of wild type in transcripts of heterozygous atr mutants and a decrease to 40% in homozygous mutants. Ratios of atr cDNA to atm cDNA as a control are shown, each normalized to the levels in the respective wild-types. Quantitative PCR was performed either at the 5' region (black bars) or 3' region (gray bars).