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Table 1 Primers for cDNA cloning, Northern blotting, semi-quantitative RT-PCR, dsRNA synthesis and detection of SeHex and SeSP1 RNAi

From: Two storage hexamerins from the beet armyworm Spodoptera exigua: Cloning, characterization and the effect of gene silencing on survival

PCR
Fragme
Primers
  Name Directiona Typeb Nucleotide Sequence (5'-3')
1 Hex-DF1 F D TGG ARM GHC TGT CYA ACG
  Hex-DF2 F D ACA AKG GMA TBH HST TCC
  SP1-DF1 F D GGC ATG TTC DTV TAT GC
  SP1-DF2 F D TAC CCN TAC TWC TTC GTC
  Hex-DR1 R D GAA BGG CAT RCC DCC GAC
  Hex-DR2 R D GTC CAG CTS SAW GAA GTT
  SP1-DR1 R D CGT ACT TGG GNC CAN DGA AG
  SP1-DR2 R D TGA ANG GVT GRT GRT TGA G
2
RACE
3-CDS R O AAG CAG TGG TAT CAA CGC AGA GTA C(T)30VN
  NUP F A AAG CAG TGG TAT CAA CGC AGA GT
  Hex-5R1 R G GCT GAT GTC AAT ACG TTC ACC GAG
  Hex-5R2 R G CGT AAC CCT GTT CGA TGG CGT CAC
  SP1-5R1 R G GAT GAC ATG GCT ATC GAC GA
  SP1-5R2 R G CGT AGG GAG CAG GCA GAG TG
  Hex-3F1 F G GTG AGC GTG TTG GTA CAG CAT CC
  Hex-3F2 F G GAG TGA AGT TGC TAA GAC CGT TC
  SP1-3F1 F G CAA CTG ACA AGC TAG TGA CC
  SP1-3F2 F G CGC TGA GAT GAA GAA GAA GC
3 probe Hex-FP F G CGT TTG CAC GAG GTC TTC C
  Hex-RP R G GGC TTC TTC TTC ATT CAT GTG C
  SP1-FP F G GAC CAA GGC AGC GAA TGA CC
  SP1-RP R G CAG GGC GTT GGT GAT ATC CA
4 dsRNA dsHex-F F G GAC AGC CAC GGC TAT GAG ATT CC
  dsHex-FT F G GGA TCC TAA TAC GAC TCA CTA TAG GNG ACA GCC ACG GCT ATG AGA TTC C
  dsHex-R R G CCT TAG AAA CAT TGG TAG GCT CG
  dsHex-RT R G GGA TCC TAA TAC GAC TCA CTA TAG GNC CTT AGA AAC ATT GGT AGG CTC G
  dsSP1-F F G GAC TGG AAA GAA CAC CAT CGT C
  dsSP1-FT F G GGA TCC TAA TAC GAC TCA CTA TAG GNG ACT GGA AAG AAC ACC ATC GTC
  dsSP1-R R G GGT CCT TCC TGT ACA CCA TCA C
  dsSP1-RT R G GGA TCC TAA TAC GAC TCA CTA TAG GNG GTC CTT CCT GTA CAC CAT CAC
  1. a F, forward, R, reverse; b D, degenerate primer; G, gene-specific primer; A, nested universal primer; O, 3'-RACE CDS primer.