Table 1 Primers for cDNA cloning, Northern blotting, semi-quantitative RT-PCR, dsRNA synthesis and detection of SeHex and SeSP1 RNAi
PCR Fragme | Primers | |||
|---|---|---|---|---|
| Â | Name | Directiona | Typeb | Nucleotide Sequence (5'-3') |
1 | Hex-DF1 | F | D | TGG ARM GHC TGT CYA ACG |
| Â | Hex-DF2 | F | D | ACA AKG GMA TBH HST TCC |
| Â | SP1-DF1 | F | D | GGC ATG TTC DTV TAT GC |
| Â | SP1-DF2 | F | D | TAC CCN TAC TWC TTC GTC |
| Â | Hex-DR1 | R | D | GAA BGG CAT RCC DCC GAC |
| Â | Hex-DR2 | R | D | GTC CAG CTS SAW GAA GTT |
| Â | SP1-DR1 | R | D | CGT ACT TGG GNC CAN DGA AG |
| Â | SP1-DR2 | R | D | TGA ANG GVT GRT GRT TGA G |
2 RACE | 3-CDS | R | O | AAG CAG TGG TAT CAA CGC AGA GTA C(T)30VN |
| Â | NUP | F | A | AAG CAG TGG TAT CAA CGC AGA GT |
| Â | Hex-5R1 | R | G | GCT GAT GTC AAT ACG TTC ACC GAG |
| Â | Hex-5R2 | R | G | CGT AAC CCT GTT CGA TGG CGT CAC |
| Â | SP1-5R1 | R | G | GAT GAC ATG GCT ATC GAC GA |
| Â | SP1-5R2 | R | G | CGT AGG GAG CAG GCA GAG TG |
| Â | Hex-3F1 | F | G | GTG AGC GTG TTG GTA CAG CAT CC |
| Â | Hex-3F2 | F | G | GAG TGA AGT TGC TAA GAC CGT TC |
| Â | SP1-3F1 | F | G | CAA CTG ACA AGC TAG TGA CC |
| Â | SP1-3F2 | F | G | CGC TGA GAT GAA GAA GAA GC |
3 probe | Hex-FP | F | G | CGT TTG CAC GAG GTC TTC C |
| Â | Hex-RP | R | G | GGC TTC TTC TTC ATT CAT GTG C |
| Â | SP1-FP | F | G | GAC CAA GGC AGC GAA TGA CC |
| Â | SP1-RP | R | G | CAG GGC GTT GGT GAT ATC CA |
4 dsRNA | dsHex-F | F | G | GAC AGC CAC GGC TAT GAG ATT CC |
| Â | dsHex-FT | F | G | GGA TCC TAA TAC GAC TCA CTA TAG GNG ACA GCC ACG GCT ATG AGA TTC C |
| Â | dsHex-R | R | G | CCT TAG AAA CAT TGG TAG GCT CG |
| Â | dsHex-RT | R | G | GGA TCC TAA TAC GAC TCA CTA TAG GNC CTT AGA AAC ATT GGT AGG CTC G |
| Â | dsSP1-F | F | G | GAC TGG AAA GAA CAC CAT CGT C |
| Â | dsSP1-FT | F | G | GGA TCC TAA TAC GAC TCA CTA TAG GNG ACT GGA AAG AAC ACC ATC GTC |
| Â | dsSP1-R | R | G | GGT CCT TCC TGT ACA CCA TCA C |
| Â | dsSP1-RT | R | G | GGA TCC TAA TAC GAC TCA CTA TAG GNG GTC CTT CCT GTA CAC CAT CAC |