L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription factors β-catenin or Slug. (A) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. (B) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. (C) Western blot analysis for L1CAM and β-catenin as well as GAP-DH, used as a loading control. (D) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.