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Table 1 Review of normalization "housekeeping" genes used for RT-qPCR analysis of mesenchymal stromal cells

From: EF1α and RPL13a represent normalization genes suitable for RT-qPCR analysis of bone marrow derived mesenchymal stem cells

RT-qPCR
Gene Full Name Reference
SMa-actin alpha-2 smooth muscle aorta actin Ross et al 2006[26]
ACTB β-actin Dickhut et al 2009 [27], Murthy et al 2008 [28]
GAPDH Glyceraldehyde-3-phosphate dehydrogenase Block et al 2009 [29], Gang et al 2009 [30], *Lu et al2009 [31], Leonardi et al 2009 [32], Schneider et al 2009
[33], Tan et al 2009 [34], Corcoran et al 2008 [35],
B2M β2-microglobulin Briquet et al 2009 [36]
Semi-quantitative or normal PCR
Gene Full Name Reference
EF1α Eukaryotic translational elongation factor 1 alpha D'Ippolito et al 2006 [15, 37, 38], D'Ippolito et al 2004 [3]
ACTB β-actin Riekstina et al 2009 [39], Gang et al 2007 [5], Shim et al 2004 [40]
GAPDH Glyceraldehyde-3-phosphate dehydrogenase Drost et al 2009 [41], Tang et al 2008 [42], Greco et al 2008 [43], Trzaska et al 2008 [44], Betrami et al 2007 [4], Greco et al 2007 [45], Tatard et al 2007 [17], Trzaska et al 2007 [46], Muguruma et al 2003 [47]
B2M β2-microglobulin Pozzobon et al 2009 [6], Martinez et al 2007 [48]
  1. * used more than one normalization gene (GAPDH and 18 S RNA)
  2. Table#1: Literature searches were conducted through PubMed Central http://www.pubmed.com. Representative manuscripts were selected which used quantitative real time PCR (RT-qPCR) or semi-quantitative PCR analyses for the analysis of gene expression in human bone marrow derived adult stem cells or classical MSC to determine commonly used normalization "housekeeping" genes.