Relative mRNA expression levels of normalization genes in MIAMI cells. MIAMI cells expanded under normal expansion conditions (3% pO2) were harvested for total RNA. Real-time RT-PCR analysis was conducted using 160 nM of both forward and reverse primers with 50 ng of cDNA. Relative fold difference was calculated using the ΔΔCt method [13, 22]. N = 8 independent experiments. HPRT1 was used as the relative control and was set to the value of 1.