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Table 2 Some predicted physico-chemical parameters of the novel ESTs identified by enrichment for dehydration-induced gene expression in A. avenae and their relative expression following exposure of the nematodes to four environmental stresses.

From: Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae

     

Relative Expression

Accession no.

No. amino acids

Predicted pI

Predicted GRAVY score

Percent disorder§

Desiccation

Osmotic

Heat

Cold

GR463919

179

9.28

-1.16

75

1.0

1.1

0.6

1.2

GR463920

98

10.51

-0.87

39

1.7

1.8

1.7

1.2

GR463921

88

4.36

0.01

78

1.8

3.4

0.5

1.0

GR463922

168

11.13

-0.55

39

1.5

1.7

1.4

2.1

GR463923

144

6.78

0.20

16

1.2

1.4

1.4

2.2

*GR463924

35

6.69

-0.13

37

4.3

10.9

3.7

8.3

GR463925

143

9.82

-0.08

22

1.5

1.4

1.2

0.5

GR463926

140

9.64

-0.97

51

8.4

4.5

35.7

6.0

GR463927

122

10.91

-0.30

37

1.0

20.6

1.5

0.5

†GR463928

37

6.33

0.28

46

9.0

14.5

0.8

2.2

GR463929

59

4.17

0.99

0

3.5

22.6

4.7

2.7

*EF026246

61

6.35

-2.32

20

1.9

2.2

1.2

2.6

  1. Where accession numbers are shown in bold, respective ESTs were used in cross-species RNAi experiments with P. superbus. Expression values in regular type represent a statistically significant upregulation in gene expression with respect to 5.8S rRNA and ama-1 genes; values in grey font are not significant. §PONDR VL-XT predictions. *The reading frame selected was from the same strand as the spliced leader sequence. †The reading frame selected was from the same strand as the polyA tail. Where such landmarks were not available the longest predicted open reading frame was used to estimate physico-chemical parameters.