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Table 2 Some predicted physico-chemical parameters of the novel ESTs identified by enrichment for dehydration-induced gene expression in A. avenae and their relative expression following exposure of the nematodes to four environmental stresses.

From: Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae

      Relative Expression
Accession no. No. amino acids Predicted pI Predicted GRAVY score Percent disorder§ Desiccation Osmotic Heat Cold
GR463919 179 9.28 -1.16 75 1.0 1.1 0.6 1.2
GR463920 98 10.51 -0.87 39 1.7 1.8 1.7 1.2
GR463921 88 4.36 0.01 78 1.8 3.4 0.5 1.0
GR463922 168 11.13 -0.55 39 1.5 1.7 1.4 2.1
GR463923 144 6.78 0.20 16 1.2 1.4 1.4 2.2
*GR463924 35 6.69 -0.13 37 4.3 10.9 3.7 8.3
GR463925 143 9.82 -0.08 22 1.5 1.4 1.2 0.5
GR463926 140 9.64 -0.97 51 8.4 4.5 35.7 6.0
GR463927 122 10.91 -0.30 37 1.0 20.6 1.5 0.5
†GR463928 37 6.33 0.28 46 9.0 14.5 0.8 2.2
GR463929 59 4.17 0.99 0 3.5 22.6 4.7 2.7
*EF026246 61 6.35 -2.32 20 1.9 2.2 1.2 2.6
  1. Where accession numbers are shown in bold, respective ESTs were used in cross-species RNAi experiments with P. superbus. Expression values in regular type represent a statistically significant upregulation in gene expression with respect to 5.8S rRNA and ama-1 genes; values in grey font are not significant. §PONDR VL-XT predictions. *The reading frame selected was from the same strand as the spliced leader sequence. †The reading frame selected was from the same strand as the polyA tail. Where such landmarks were not available the longest predicted open reading frame was used to estimate physico-chemical parameters.