Experimental comparison of relative RT-qPCR quantification approaches for gene expression studies in poplar

BMC Molecular Biology

Table 1 Primer sequences

Gene	Forward primer	Reverse primer	Amplicon size (bp)
Reference genes
ACT	ACC CTC CAA TCC AGA CAC TG	TTG CTG ACC GTA TGA GCA AG	105
EF1	AAG CCA TGG GAT GAT GAG AC	ACT GGA GCC AAT TTT GAT GC	101
UBQ	CGT GGA GGA ATG CAG ATT TT	GAT CTT GGC CTT CAC GTT GT	99
Target genes
APX	TCT TGC GAG GAA GTG AAG GT	AAT GGT TGG ACC TCC AGT GA	100
SOD	GGG TCT CGT CCA ACA CAC TT	AGC CAT GGC GAT AGA TTG AC	96

Primer sequences and amplicon sizes for the reference genes ACT, EF1 and UBQ and the target genes APX and SOD [14].

ISSN: 1471-2199