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Table 1 The activities of the constructs isolated as measured in three reporter systems.

From: A method for selecting cis-acting regulatory sequences that respond to small molecule effectors

Erythromycin activity in reporter system
name amino acid sequence lactamase galactosidase GFP
E1 Asp His Tyr Ser Arg Ile Val 2.02 (±0.11) 1.46 (±0.10) ND
E2 Val Ile Gly Arg Asn Ser Phe 1.70 (±0.09) 1.85 (±0.04) ND
E3 Gly Thr Ser Leu Gly Arg Gly 1.66 (±0.09) 1.61 (±0.08) ND
E4 Leu Gly Ile Arg Ser Gly Tyr 1.63 (±0.11) 2.04 (±0.03) 1.62 (±0.12)
E5 Ile Ser Val Trp Leu Val Ala 1.58 (±0.16) 1.95 (±0.05) 1.55 (±0.01)
E6 Gly Trp His Leu Arg Leu Val 1.56 (±0.14) 1.95 (±0.06) 1.33 (±0.05)
E7 Val Gly Leu Phe Ala Asn Gly 1.54 (±0.12) 1.53 (±0.08) ND
E8 Ala Ser Ser Val Ser His Leu 1.53 (±0.21) 1.73 (±0.12) ND
E9 Phe Ile Leu Ser Ile Ser Leu 1.48 (±0.17) 1.63 (±0.06) ND
E10 Leu Val Leu Cys Asn Leu Leu 1.46 (±0.07) 1.41 (±0.04) ND
control - 1.06 (±0.13) 1.26 (±0.06) 1.15 (±0.04)
Chloramphenicol activity in reporter system
name amino acid sequence lactamase galactosidase GFP
C1 Gln Glu Arg Ala Thr Gly Val 3.06 (±0.18) 2.46 (±0.06) 1.95 (±0.02)
C2 Arg Gly Gly Phe Ser Asn Glu 2.89 (±0.06) 1.73 (±0.08) ND
C3 Glu Asn Ala Pro Ser Tyr Asp 2.62 (±0.10) 2.00 (±0.14) ND
C4 Ser Ser Gly Ala Phe Gly Thr 2.49 (±0.04) 1.74 (±0.12) ND
C5 Arg Val Pro Pro Arg Arg Cys 2.35 (±0.14) 2.08 (±0.09) ND
C6 Phe Leu Glu Val Gly Cys Ser 2.32 (±0.11) 2.40 (±0.03) 3.11 (±0.02)
C7 Gly Arg Gln Val Gly Arg Val 2.14 (±0.10) 1.72 (±0.11) ND
C8 Ser Val Cys Cys Phe Val Phe 2.01 (±0.08) 2.05 (±0.13) ND
C9 Cys Trp Gly Ile Asp Met Ala 2.00 (±0.15) 2.17 (±0.04) 1.91 (±0.05)
C10 Lys Thr Tyr Val Gly Lys Ile 1.93 (±0.12) 1.95 (±0.07) ND
control - 1.41 (±0.06) 1.43 (±0.12) 1.43 (±0.08)
Troleandomycin activity in reporter system
name amino acid sequence lactamase galactosidase GFP
T1 Asn Leu Glu Ser Val Leu Ser 2.03 (±0.14) 2.36 (±0.11) 0.46 (±0.02)
T2 Arg Val Arg Asp Val Phe Tyr 1.78 (±0.05) 1.53 (±0.10) ND
T3 Arg Gly Glu Gly Trp Gly Leu 1.67 (±0.15) 1.63 (±0.14) ND
T4 Gly Arg Gly Arg Cys Val Ser 1.65 (±0.06) 2.14 (±0.10) 0.70 (±0.21)
T5 Ser Ala Glu Ala Phe Arg Ala 1.58 (±0.08) 1.51 (±0.07) ND
T6 Ile Leu Met Leu Arg Arg Ser 1.56 (±0.13) 1.62 (±0.11) ND
T7 Gly Gly Ser Ala Phe Ile Glu 1.45 (±0.13) 1.53 (±0.04) ND
T8 Glu Cys Gln Ser Arg His Met 1.37 (±0.10) 1.67 (±0.08) ND
T9 Ile Ser Ile Met Arg Arg Cys 1.21 (±0.08) 1.85 (±0.06) 0.81 (±0.10)
T10 Pro Ser Ala Arg Ile His Arg 1.20 (±0.11) 1.38 (±0.13) ND
control - 1.11 (±0.13) 1.54 (±0.03) 0.91 (±0.05)
Meta-toluate activity in reporter system
name amino acid sequence lactamase galactosidase GFP
M1 Glu Arg Thr Tyr Ile Met Tyr 2.35 (±0.13) 0.99 (±0.08) 1.34 (±0.07)
M2 Ser Trp Gly Leu Ala Ala Lys 2.24 (±0.08) 0.73 (±0.09) 1.39 (±0.06)
M3 Arg Ala Asp Arg Phe Lys Phe 2.14 (±0.04) 1.10 (±0.01) 1.86 (±0.15)
M4 Lys Tyr Gln Leu Leu Ile Cys 1.90 (±0.12) 1.21 (±0.06) 1.24 (±0.05)
M5 Gly Trp His Arg Trp Trp Tyr 1.84 (±0.06) 0.97 (±0.06) 1.26 (±0.04)
M6 Gly Lys Phe Gly Ser Ala Ser 1.81 (±0.11) 1.04 (±0.05) 1.38 (±0.12)
M7 Leu Val Val Thr Thr Leu Ile 1.80 (±0.08) 0.89 (±0.04) 1.69 (±0.15)
M8 Gly Lys Leu Tyr Arg Tyr Asp 1.75 (±0.05) 0.98 (±0.03) 1.65 (±0.13)
M9 Ile Ser Val Trp Leu Val Ala 1.67 (±0.05) 0.94 (±0.09) 0.97 (±0.05)
M10 Ser Arg Val Leu Val Ser Cys 1.64 (±0.06) 1.06 (±0.02) 2.23 (±0.22)
control - 1.34 (±0.04) 0.89 (±0.11) 1.63 (±0.05)
HSL   activity in reporter system
name amino acid sequence lactamase galactosidase GFP
H1 Thr Lys Leu Ser Pro Leu Ile 1.44 (±0.11) ND 1.23 (±0.06)
H2 Ala Leu Pro Val Val Val Ile 1.39 (±0.05) ND 1.49 (±0.06)
H3 Gly Ala Val Asp Val Leu Val 1.37 (±0.13) ND 1.24 (±0.12)
H4 Asp Tyr Val Asp Cys Pro Arg 1.35 (±0.06) ND 1.44 (±0.04)
H5 Phe Leu Cys Pro His Asp Ser 1.33 (±0.08) ND 1.18 (±0.02)
H6 Glu Leu Phe Val Pro Tyr Cys 1.23 (±0.07) ND ND
H7 Lys Ala Ser Leu Asp Pro Leu 1.21 (±0.09) ND ND
H8 Gly Ala Phe Met Val Asp Ser 1.13 (±0.07) ND ND
H9 Asn Ala Ile Ala Ile Arg Arg 1.12 (±0.15) ND 1.56 (±0.11)
H10 Asn Val Leu Ala Phe Lys Gly 1.11 (±0.10) ND 1.26 (±0.04)
control - 0.96 (±0.11) ND 1.32 (±0.05)
  1. The activity is shown as a ratio of marker gene expression measured in the absence and presence of the corresponding compound. The plasmids without 5' extensions in the marker gene coding ORFs, p177-NotI, pPOTZ-HindIII and pETGFP, were used as controls for β-lactamase, β-galactosidase and GFP activity respectively. Amino acid sequences of the peptides encoded by the randomized part of the plasmid are shown. Standard errors are indicated. ND - not determined.