Table 1 The activities of the constructs isolated as measured in three reporter systems.
From: A method for selecting cis-acting regulatory sequences that respond to small molecule effectors
Erythromycin | activity in reporter system | |||
|---|---|---|---|---|
name | amino acid sequence | lactamase | galactosidase | GFP |
E1 | Asp His Tyr Ser Arg Ile Val | 2.02 (±0.11) | 1.46 (±0.10) | ND |
E2 | Val Ile Gly Arg Asn Ser Phe | 1.70 (±0.09) | 1.85 (±0.04) | ND |
E3 | Gly Thr Ser Leu Gly Arg Gly | 1.66 (±0.09) | 1.61 (±0.08) | ND |
E4 | Leu Gly Ile Arg Ser Gly Tyr | 1.63 (±0.11) | 2.04 (±0.03) | 1.62 (±0.12) |
E5 | Ile Ser Val Trp Leu Val Ala | 1.58 (±0.16) | 1.95 (±0.05) | 1.55 (±0.01) |
E6 | Gly Trp His Leu Arg Leu Val | 1.56 (±0.14) | 1.95 (±0.06) | 1.33 (±0.05) |
E7 | Val Gly Leu Phe Ala Asn Gly | 1.54 (±0.12) | 1.53 (±0.08) | ND |
E8 | Ala Ser Ser Val Ser His Leu | 1.53 (±0.21) | 1.73 (±0.12) | ND |
E9 | Phe Ile Leu Ser Ile Ser Leu | 1.48 (±0.17) | 1.63 (±0.06) | ND |
E10 | Leu Val Leu Cys Asn Leu Leu | 1.46 (±0.07) | 1.41 (±0.04) | ND |
control | - | 1.06 (±0.13) | 1.26 (±0.06) | 1.15 (±0.04) |
Chloramphenicol | activity in reporter system | |||
name | amino acid sequence | lactamase | galactosidase | GFP |
C1 | Gln Glu Arg Ala Thr Gly Val | 3.06 (±0.18) | 2.46 (±0.06) | 1.95 (±0.02) |
C2 | Arg Gly Gly Phe Ser Asn Glu | 2.89 (±0.06) | 1.73 (±0.08) | ND |
C3 | Glu Asn Ala Pro Ser Tyr Asp | 2.62 (±0.10) | 2.00 (±0.14) | ND |
C4 | Ser Ser Gly Ala Phe Gly Thr | 2.49 (±0.04) | 1.74 (±0.12) | ND |
C5 | Arg Val Pro Pro Arg Arg Cys | 2.35 (±0.14) | 2.08 (±0.09) | ND |
C6 | Phe Leu Glu Val Gly Cys Ser | 2.32 (±0.11) | 2.40 (±0.03) | 3.11 (±0.02) |
C7 | Gly Arg Gln Val Gly Arg Val | 2.14 (±0.10) | 1.72 (±0.11) | ND |
C8 | Ser Val Cys Cys Phe Val Phe | 2.01 (±0.08) | 2.05 (±0.13) | ND |
C9 | Cys Trp Gly Ile Asp Met Ala | 2.00 (±0.15) | 2.17 (±0.04) | 1.91 (±0.05) |
C10 | Lys Thr Tyr Val Gly Lys Ile | 1.93 (±0.12) | 1.95 (±0.07) | ND |
control | - | 1.41 (±0.06) | 1.43 (±0.12) | 1.43 (±0.08) |
Troleandomycin | activity in reporter system | |||
name | amino acid sequence | lactamase | galactosidase | GFP |
T1 | Asn Leu Glu Ser Val Leu Ser | 2.03 (±0.14) | 2.36 (±0.11) | 0.46 (±0.02) |
T2 | Arg Val Arg Asp Val Phe Tyr | 1.78 (±0.05) | 1.53 (±0.10) | ND |
T3 | Arg Gly Glu Gly Trp Gly Leu | 1.67 (±0.15) | 1.63 (±0.14) | ND |
T4 | Gly Arg Gly Arg Cys Val Ser | 1.65 (±0.06) | 2.14 (±0.10) | 0.70 (±0.21) |
T5 | Ser Ala Glu Ala Phe Arg Ala | 1.58 (±0.08) | 1.51 (±0.07) | ND |
T6 | Ile Leu Met Leu Arg Arg Ser | 1.56 (±0.13) | 1.62 (±0.11) | ND |
T7 | Gly Gly Ser Ala Phe Ile Glu | 1.45 (±0.13) | 1.53 (±0.04) | ND |
T8 | Glu Cys Gln Ser Arg His Met | 1.37 (±0.10) | 1.67 (±0.08) | ND |
T9 | Ile Ser Ile Met Arg Arg Cys | 1.21 (±0.08) | 1.85 (±0.06) | 0.81 (±0.10) |
T10 | Pro Ser Ala Arg Ile His Arg | 1.20 (±0.11) | 1.38 (±0.13) | ND |
control | - | 1.11 (±0.13) | 1.54 (±0.03) | 0.91 (±0.05) |
Meta-toluate | activity in reporter system | |||
name | amino acid sequence | lactamase | galactosidase | GFP |
M1 | Glu Arg Thr Tyr Ile Met Tyr | 2.35 (±0.13) | 0.99 (±0.08) | 1.34 (±0.07) |
M2 | Ser Trp Gly Leu Ala Ala Lys | 2.24 (±0.08) | 0.73 (±0.09) | 1.39 (±0.06) |
M3 | Arg Ala Asp Arg Phe Lys Phe | 2.14 (±0.04) | 1.10 (±0.01) | 1.86 (±0.15) |
M4 | Lys Tyr Gln Leu Leu Ile Cys | 1.90 (±0.12) | 1.21 (±0.06) | 1.24 (±0.05) |
M5 | Gly Trp His Arg Trp Trp Tyr | 1.84 (±0.06) | 0.97 (±0.06) | 1.26 (±0.04) |
M6 | Gly Lys Phe Gly Ser Ala Ser | 1.81 (±0.11) | 1.04 (±0.05) | 1.38 (±0.12) |
M7 | Leu Val Val Thr Thr Leu Ile | 1.80 (±0.08) | 0.89 (±0.04) | 1.69 (±0.15) |
M8 | Gly Lys Leu Tyr Arg Tyr Asp | 1.75 (±0.05) | 0.98 (±0.03) | 1.65 (±0.13) |
M9 | Ile Ser Val Trp Leu Val Ala | 1.67 (±0.05) | 0.94 (±0.09) | 0.97 (±0.05) |
M10 | Ser Arg Val Leu Val Ser Cys | 1.64 (±0.06) | 1.06 (±0.02) | 2.23 (±0.22) |
control | - | 1.34 (±0.04) | 0.89 (±0.11) | 1.63 (±0.05) |
HSL | activity in reporter system | |||
name | amino acid sequence | lactamase | galactosidase | GFP |
H1 | Thr Lys Leu Ser Pro Leu Ile | 1.44 (±0.11) | ND | 1.23 (±0.06) |
H2 | Ala Leu Pro Val Val Val Ile | 1.39 (±0.05) | ND | 1.49 (±0.06) |
H3 | Gly Ala Val Asp Val Leu Val | 1.37 (±0.13) | ND | 1.24 (±0.12) |
H4 | Asp Tyr Val Asp Cys Pro Arg | 1.35 (±0.06) | ND | 1.44 (±0.04) |
H5 | Phe Leu Cys Pro His Asp Ser | 1.33 (±0.08) | ND | 1.18 (±0.02) |
H6 | Glu Leu Phe Val Pro Tyr Cys | 1.23 (±0.07) | ND | ND |
H7 | Lys Ala Ser Leu Asp Pro Leu | 1.21 (±0.09) | ND | ND |
H8 | Gly Ala Phe Met Val Asp Ser | 1.13 (±0.07) | ND | ND |
H9 | Asn Ala Ile Ala Ile Arg Arg | 1.12 (±0.15) | ND | 1.56 (±0.11) |
H10 | Asn Val Leu Ala Phe Lys Gly | 1.11 (±0.10) | ND | 1.26 (±0.04) |
control | - | 0.96 (±0.11) | ND | 1.32 (±0.05) |