Figure 3

Cross-responses of the sequences to different compounds as measured in the β-galactosidase reporter system. The sequences originally selected on erythromycin (A), chloramphenicol (B), troleandomycin (C) and meta-toluate (D) were tested. Constructs containing ErmC leader peptide and a mutant of E4 peptide (Mut8, Figure 2) were included. The expression of β-galactosidase marker protein was measured 3 h after induction with IPTG in the absence and presence of compound (E-erythromycin, C-chloramphenicol, T-troleandomycin, M-meta-toluate). The activity of each construct is shown as a ratio of Miller units measured in the absence and presence of the compound. Gray bars indicate the activity in the presence of the compound which was used for selecting the sequence.