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Table 3 Primer sequence, melting temperature and the expected amplification product of PmSap and PmDmc1

From: Identification of testis-relevant genes using in silico analysis from testis ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon)

Gene/Primer

Sequence

Tm (°C)

Size (bp)

RACE-PCR

   

Saposin

   

   5'-RACE-1

5'-CTGGCACCAATAAGAAGGACCCCAAGTG-3'

86

-

   5'-RACE-2

5'-CAGATGGGCAGAGATGCAGCATAGGACA-3'

86

-

Dmc1

   

   5'-RACE

5'-GCCGCCAATCGGTTTCTTCGGGTCC-3'

82

-

   3'-RACE

5'-CCTTCGCTATCACAGCAGGAGGCATTG-3'

84

-

Real-time PCR

   

Saposin

   

Standard curve

F: 5'-GCTATGGTTCAGGTTGATGACTTGC-3'

74

614

 

R: 5'-ACTCCCTTCCACACCTTCGTTTC-3'

70

 

Real-time PCR

F: 5'-CCATAAAGTTCTGCCCCCACCAC-3'

68

145

 

R: 5'-CCCTTCCACACCTTCGTTTCACA-3'

70

 

EF-1α

   

Standard curve

F: 5'-GCTCTTACCGAGGCTGTCCC-3'

66

434

 

R: 5'-GTGGGTGTAATCAAGGAGGTCAA-3'

68

 

Real-time PCR

F: 5'-TTCCGACTCCAAGAACGACC-3'

62

122

 

R: 5'-GAGCAGTGTGGCAATCAAGC-3'

62

 

Dmc1

   

Standard curve

F: 5'-ATGGAAGATCAGGCTTTAGATGC-3'

66

425

 

R: 5'-GTGACGCAGAGAGTGTGGGAG-3'

68

 

Real-time PCR

F: 5'-ATGTGCGAGAAGCGAAGGC-3'

60

150

 

R: 5'-GCAGAGAGTGTGGGAGATTTGTG-3'

70