Table 3 Primer sequence, melting temperature and the expected amplification product of PmSap and PmDmc1
Gene/Primer | Sequence | Tm (°C) | Size (bp) |
|---|---|---|---|
RACE-PCR | Â | Â | Â |
Saposin | Â | Â | Â |
   5'-RACE-1 | 5'-CTGGCACCAATAAGAAGGACCCCAAGTG-3' | 86 | - |
   5'-RACE-2 | 5'-CAGATGGGCAGAGATGCAGCATAGGACA-3' | 86 | - |
Dmc1 | Â | Â | Â |
   5'-RACE | 5'-GCCGCCAATCGGTTTCTTCGGGTCC-3' | 82 | - |
   3'-RACE | 5'-CCTTCGCTATCACAGCAGGAGGCATTG-3' | 84 | - |
Real-time PCR | Â | Â | Â |
Saposin | Â | Â | Â |
Standard curve | F: 5'-GCTATGGTTCAGGTTGATGACTTGC-3' | 74 | 614 |
| Â | R: 5'-ACTCCCTTCCACACCTTCGTTTC-3' | 70 | Â |
Real-time PCR | F: 5'-CCATAAAGTTCTGCCCCCACCAC-3' | 68 | 145 |
| Â | R: 5'-CCCTTCCACACCTTCGTTTCACA-3' | 70 | Â |
EF-1α |  |  |  |
Standard curve | F: 5'-GCTCTTACCGAGGCTGTCCC-3' | 66 | 434 |
| Â | R: 5'-GTGGGTGTAATCAAGGAGGTCAA-3' | 68 | Â |
Real-time PCR | F: 5'-TTCCGACTCCAAGAACGACC-3' | 62 | 122 |
| Â | R: 5'-GAGCAGTGTGGCAATCAAGC-3' | 62 | Â |
Dmc1 | Â | Â | Â |
Standard curve | F: 5'-ATGGAAGATCAGGCTTTAGATGC-3' | 66 | 425 |
| Â | R: 5'-GTGACGCAGAGAGTGTGGGAG-3' | 68 | Â |
Real-time PCR | F: 5'-ATGTGCGAGAAGCGAAGGC-3' | 60 | 150 |
| Â | R: 5'-GCAGAGAGTGTGGGAGATTTGTG-3' | 70 | Â |