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Table 3 Primer sequence, melting temperature and the expected amplification product of PmSap and PmDmc1

From: Identification of testis-relevant genes using in silico analysis from testis ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon)

Gene/Primer Sequence Tm (°C) Size (bp)
RACE-PCR    
Saposin    
   5'-RACE-1 5'-CTGGCACCAATAAGAAGGACCCCAAGTG-3' 86 -
   5'-RACE-2 5'-CAGATGGGCAGAGATGCAGCATAGGACA-3' 86 -
Dmc1    
   5'-RACE 5'-GCCGCCAATCGGTTTCTTCGGGTCC-3' 82 -
   3'-RACE 5'-CCTTCGCTATCACAGCAGGAGGCATTG-3' 84 -
Real-time PCR    
Saposin    
Standard curve F: 5'-GCTATGGTTCAGGTTGATGACTTGC-3' 74 614
  R: 5'-ACTCCCTTCCACACCTTCGTTTC-3' 70  
Real-time PCR F: 5'-CCATAAAGTTCTGCCCCCACCAC-3' 68 145
  R: 5'-CCCTTCCACACCTTCGTTTCACA-3' 70  
EF-1α    
Standard curve F: 5'-GCTCTTACCGAGGCTGTCCC-3' 66 434
  R: 5'-GTGGGTGTAATCAAGGAGGTCAA-3' 68  
Real-time PCR F: 5'-TTCCGACTCCAAGAACGACC-3' 62 122
  R: 5'-GAGCAGTGTGGCAATCAAGC-3' 62  
Dmc1    
Standard curve F: 5'-ATGGAAGATCAGGCTTTAGATGC-3' 66 425
  R: 5'-GTGACGCAGAGAGTGTGGGAG-3' 68  
Real-time PCR F: 5'-ATGTGCGAGAAGCGAAGGC-3' 60 150
  R: 5'-GCAGAGAGTGTGGGAGATTTGTG-3' 70  
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