|
Saposin
| | | |
|
5'-RACE-1
|
5'-CTGGCACCAATAAGAAGGACCCCAAGTG-3'
|
86
|
-
|
|
5'-RACE-2
|
5'-CAGATGGGCAGAGATGCAGCATAGGACA-3'
|
86
|
-
|
|
Dmc1
| | | |
|
5'-RACE
|
5'-GCCGCCAATCGGTTTCTTCGGGTCC-3'
|
82
|
-
|
|
3'-RACE
|
5'-CCTTCGCTATCACAGCAGGAGGCATTG-3'
|
84
|
-
|
|
Real-time PCR
| | | |
|
Saposin
| | | |
|
Standard curve
|
F: 5'-GCTATGGTTCAGGTTGATGACTTGC-3'
|
74
|
614
|
| |
R: 5'-ACTCCCTTCCACACCTTCGTTTC-3'
|
70
| |
|
Real-time PCR
|
F: 5'-CCATAAAGTTCTGCCCCCACCAC-3'
|
68
|
145
|
| |
R: 5'-CCCTTCCACACCTTCGTTTCACA-3'
|
70
| |
|
EF-1α
| | | |
|
Standard curve
|
F: 5'-GCTCTTACCGAGGCTGTCCC-3'
|
66
|
434
|
| |
R: 5'-GTGGGTGTAATCAAGGAGGTCAA-3'
|
68
| |
|
Real-time PCR
|
F: 5'-TTCCGACTCCAAGAACGACC-3'
|
62
|
122
|
| |
R: 5'-GAGCAGTGTGGCAATCAAGC-3'
|
62
| |
|
Dmc1
| | | |
|
Standard curve
|
F: 5'-ATGGAAGATCAGGCTTTAGATGC-3'
|
66
|
425
|
| |
R: 5'-GTGACGCAGAGAGTGTGGGAG-3'
|
68
| |
|
Real-time PCR
|
F: 5'-ATGTGCGAGAAGCGAAGGC-3'
|
60
|
150
|
| |
R: 5'-GCAGAGAGTGTGGGAGATTTGTG-3'
|
70
| |
