Figure 7
Analysis of Penaeus monodon Dmc1 (PmDmc1) . (A) The full-length cDNA and deduced protein sequences of Dmc1 (1,661 bp; ORF 1,026 bp, 341aa) of P. monodon. Start (ATG) and stop (TAA) codons are illustrated in boldface and underlined. The deduced DMC1 protein contains an HhH domain (positions 46-75, E-value = 2.7e-02; highlighted)) and an AAA domain (positions 118-308, E-value = 9.73e-06; highlighted). (B) A bootstrapped neighbor-joining tree illustrating phylogentic relationships of Dmc1 of various taxa. Values at the node represent the percentage of times that the particular node occurred in 1000 trees generated by bootstrapping the original aligned protein sequences. Protein sequences of different isoforms of Dmc1 from Homo sapiens (HS-Dmc1, NM_007068), Equus caballus (EC-Dmc1, XM_001501584), Macaca mulatta (MM-Dmc1, XM_001094012), Pan troglodytes (PT-Dmc1, XM_515130), Canis lupus familiaris (CLF-Dmc1-1, XM_844891 and CLF-Dmc1-2, XM_855217), Gallus gallus (GG-Dmc1, XM_425477), Mus musculus (MM-Dmc1, NM_010059), Anguilla japonica (AJ-Dmc1, AB182645), Oreochromis niloticus (ON-Dmc1, AB182646), Danio rerio (DR-Dmc1, NM_001020782), Carassius auratus (CA-Dmc1, EF545136), Bombyx mori (BM-Dmc1, NM_001044087), Hydra vulgaris (HV-Dmc1, AB047581) and Strongylocentrotus purpuratus (SP-Dmc1, XM_786187), were retrieved from GenBank and compared with Dmc1 of P. monodon (PmDmc1).