Table 2 Primers and probes used

5'RACE PRIMERS

5'RACE-adapter outer primer Fwd

5'-GCTGATGGCGATGAATGAACACTG-3'

ACVRL1 specific outer primer Rev

5'-GGGAGAGTCCAGTCTCATCCTGAA-3'

5'RACE-adapter inner primer Fwd

5'-CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG-3'

ACVRL1 specific inner primer Rev

5'-GCAGTAGTGGTTGACGAACTCGGT-3'

pALK1 CLONING PRIMERS

Cloning primer (-898/-880) Fwd

5'-GAAGCCATTCTGCTTCCC-3'

Cloning primer (-587/-569) Fwd

5'-ACAAATGGGGGACGAAGG-3'

Cloning primer (-422/-404) Fwd

5'-AAGGATAGGTAGTGTCCC-3'

Cloning primer (-284/-263) Fwd

5'-ACGTTGCCTACAGTCTCG-3'

Cloning primer (+42/+59) Rev

5'-ATTCCAGCGTCTTCCTGC-3'

PCR PRIMERS

hALK1 primer Fwd

5'-ATCTGAGCAGGGCGACAGC-3'

hALK1 primer Rev

5'-ACTCCCTGTGGTGCAGTCA-3'

h18S primer Fwd

5'-CTCAACACGGGAAACCTCAC-3'

h18S primer Rev

5'-CGCTCCACCAACTAAGAACG-3'

hSp1 primer Fwd

5'-TGCAGCAGAATTGAGTCACC-3'

hSp1 primer Rev

5'-TTGGTACTGCTGCCACTCTG-3'

hGAPDH primer Fwd

5'-AGCCACATCGCTCAGACAC-3'

hGAPDH primer Rev

5'-GCCCAATACGACCAAATCC-3'

hId1 primer Fwd

5'-GCTGCTCTACGACATGAACG-3'

hId1 primer Rev

5'-CTCCAACTGAAGGTCCCTGA-3'

ChIP PRIMERS

pALK1 First region primer (-1032/-1012) Fwd

5'-CCAGAAGGCTAGGACTAAGA-3'

pALK1 First region primer (-844/-864) Rev

5'-CTCCCTGGAACTCTGCTGAC-3'

pALK1 Second region primer (-864/-844) Fwd

5'-GTCAGCAGAGTTCCAGGGAG-3'

pALK1 Second region primer (-662/-682) Rev

5'-TTAGCCCTGAGGATGGTTTG-3'

pALK1 Third region primer (-510/-490) Fwd

5'-AAAACGCATCTGGATTTTGC-3'

pALK1 Third region primer (-260/-280) Rev

5'-GAGCCGAGACTGTAGGCAAC-3'

pALK1 Fourth region primer (-200/-180) Fwd

5'-CCCACGGCCTGAGTCCAAGG-3'

pALK1 Fourth region primer (+20/+40) Rev

5'-GGCCCAGCTCCTCCACTCC-3'

pEPO primer Fwd (-66/-42)

5'-AGCCTCTCCCCCACCCCCAGCCCGGCG-3'

pEPO primer Rev (+31/+9)

5'-CAGCCCGCGAGTACTCACCGTG-3'

EMSA PROBES

Sp1 sites WT pALK1 (-89/-56) Fwd

5'-CTGAGGGGCTGGGAGCGGCGCGGGGGTGGGTCCC-3'

Sp1 sites WT pALK1 (-89/-56) Rev

5'-GGGACCCACCCCCGCGCCGCTCCCAGCCCCTCAG-3'

Sp1 site MutA -84/-78 pALK1 Fwd

5'-CTGAGTTTT TT GGAGCGGCGCGGGGGTGGGTCCC-3'

Sp1 site MutA -84/-78 pALK1 Rev

5'-GGGACCCACCCCCGCGCCGCTCCA AAAAA CTCAG-3'

Sp1 site MutB -67/-62 pALK1 Fwd

5'-CTGAGGGGCTGGGAGCGGCGCGTTTTTTT GTCCC-3'

Sp1 site MutB -67/-62 pALK1 Rev

5'-GGGACAAAAAAA CGCGCCGCTCCCAGCCCCTCAG-3'

pENG (-50 and -24 sites) Fwd

5'-GCAGGCGGCCTGGGCCCAGCCCCTTCTC-3'

pENG (-50 and -24 sites) Rev

5'-GAGAAGGGGCTGGGCCCAGGCCGCCTGC-3'

pALK1 (-823/-795) Fwd

5'-TAAAGGTCCATTTGCTGGGGTGGGGGCCC-3'

pALK1 (-823/-795) Rev

5'-GGGCCCCCACCCCAGCAAATGGACCTTTA-3'

AATT probe Fwd

5'-AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-3'

AATT probe Rev

5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-3'