Figure 4

Sp1 consensus binding sites located along the human ACVRL1 promoter and alignment with other mammalian species. (A) Schematic representation of the 14 Sp1 consensus sites found in silico along the -1,035/+210 fragment of ACVRL1 promoter sequence. Red arrows show the newly described TSSs. The blue arrow indicates the already described TSS (+ 1). (B) Alignment with other mammalian species (source of sequences as in Figure 3). Numbers are referred to the human sequence and range from -1,035 to + 210 bp. Asterisks indicate the totally conserved residues across species. Alignments of the putative regulatory sites are shown in grey background. All the human nucleotides selected for the alignment (grey background) have a Ci-value > 60. Ci = Consensus index value. It represents the degree of conservation of each position within the matrix. Putative core sequences for the binding of Sp1 are underlined. The core sequence (usually 4 residues) of a matrix is defined as the most conserved with the theoretical consensus. Additional information about each site can be found in Table 1.