Figure 2

Nucleotide sequence of the 5'-flanking region of the ACVRL1 gene and in silico analysis of the putative transcription factor binding sites. Red arrows show the newly described TSSs (-510 and -470). The blue arrow indicates the already described TSS (+ 1). The sequence lacks TATA and CAAT boxes. Putative transcription factor binding motifs for AP1 (Activator protein 1), AP4 (Activator protein 4), CEBP (CCAAT/Enhancer Binding protein), E2F (Elongation Factor 2), EKLF/KLF1 (Krüppel-Like Factor 1), ETS (E26-Transformation Specific transcription factor), GKLF/KLF4 (Krüppel-Like Factor 4), HIF (Hypoxia Inducible Factor), NFκB (Nuclear Factor of kappa light polypeptide gene enhancer in B-cells), RXR (Retinoid X Receptor), Smad (Sma and Mad-related factor), Sp1 and ZF9/KLF6 (Krüppel-Like Factor 6) are indicated by boxes. The core consensus sequences for each factor are in bold. A large number of Sp1 consensus binding sites are present in the sequence. Data were generated using the Genomatix MatInspector software tool.