Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

Table 3 Candidate reference gene inter- and intragroup variation (conrol vs. induced specimens).

Gene name	Stability value	Intergroup variation		Intragroup variation
		Induced	Control	Induced	Control
Xbp1	0.079	0.037	-0.037	0.02	0.005
Tbp	0.092	0.019	-0.019	0.06	0.014
CAPON	0.101	0.008	-0.008	0.163	0.004
Stx16	0.175	-0.121	0.121	0.062	0.001
aTub	0.337	-0.202	0.202	0.409	0.046
GAPDH	0.338	-0.217	0.217	0.128	0.124
MMP	0.602	0.476	-0.476	0.742	0.468
Best gene				Xbp1
Stability value				0.079
Best combination of two genes				Tbp and Xbp1
Stability value for best combination of two genes				0.075

Ranking according to expression stability calculated with NormFinder (NormFinder I analysis)

ISSN: 1471-2199