Figure 5
From: High-affinity consensus binding of target RNAs by the STAR/GSG proteins GLD-1, STAR-2 and Quaking
EMSA analysis of Qk-1-STAR binding to RNA fragments from the 3' UTR of MBP RNA. The wild-type sequence (MBP:2 (WT)) contains both 5' and 3' consensus sequences, underlined in black. Nucleotide changes in either the 5' (MBP:2-5'm1 or m2) or 3' (MBP:2-3'm1 or m2) consensus sites are displayed in the mutant sequences by underlining in red text. In each case, Qk-1-STAR was titrated into a reaction mixture with radiolabeled MBP RNA. The bound complex and free RNA bands are indicated with binding dissociation constants listed for each RNA. Kd values were calculated independently for each distinct shift (K1 and K2) for WT and 3'm2 RNAs.