Figure 3
From: High-affinity consensus binding of target RNAs by the STAR/GSG proteins GLD-1, STAR-2 and Quaking
STAR-2 RNA binding affinity in C21A consensus background. A. Direct titration of the STAR-2 STAR Domain into radiolabeled C21A TGE RNA by EMSA with Kd and Hill coefficient values displayed. B. Fluorescence-polarization used to measure binding of the STAR-2 STAR Domain to C21A TGE RNA. C. Competition fluorescence-polarization for 12-mer point-mutants in the C21A background. The 28-nt C21A TGE RNA was used as a probe with traces for A21 half-site RNA and four mutants shown. D. Relative IC50 values for mutant 12-mer RNAs compared to A21 half-site RNA in the A21 consensus background.