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Table 7 20 most stably expressed of the "most representative" probe sets for housekeeping genes across brain regions of normally menstruating macaques

From: Microarray analysis of relative gene expression stability for selection of internal reference genes in the rhesus macaque brain

Cycle study: brain region

rank/544

stability value

gene symbol

probe set ID

exp

1

0.053

CALM1/CALM2/CALM3

207243_s_at

++++

2

0.060

GPX4

201106_at

+++

3

0.064

COX7C

201134_x_at

++

4

0.068

COPS6

201405_s_at

+++

5

0.069

NDUFS5/RPL10

201757_at

++

6

0.071

VPS72

202261_at

++

7

0.071

RPS27A/UBB/UBC

200633_at

++++

8

0.073

ATP6V1F

201527_at

+++

9

0.074

SSR2

200652_at

++

10

0.075

PPP2CB

201375_s_at

+++

11

0.076

HINT1

207721_x_at

+++

12

0.076

CSNK2B/LY6G5B

201390_s_at

+++

13

0.078

PFDN5

207132_x_at

+++

14

0.078

SPAG7

200053_at

++

15

0.078

RPL10

200725_x_at

+++

16

0.078

GP2

206681_x_at

-

17

0.080

RPLP1

200763_s_at

+++

18

0.081

UQCRH

202233_s_at

+++

19

0.081

COX7A2L

201256_at

+++

20

0.083

ATF4

200779_at

++

Cycle study: cycle phase

rank/544

stability value

gene symbol

probe set ID

exp

1

0.026

CSNK2B/LY6G5B

201390_s_at

+++

2

0.027

RPS27A/UBB/UBC

200633_at

++++

3

0.027

HINT1

207721_x_at

+++

4

0.027

COPS6

201405_s_at

+++

5

0.028

PPP2CB

201375_s_at

+++

6

0.028

COX7C

201134_x_at

++

7

0.029

ATP6V1F

201527_at

+++

8

0.030

HAX1

201145_at

++

9

0.031

GPX4

201106_at

+++

10

0.031

CALM1/CALM2/CALM3

207243_s_at

++++

11

0.031

SPAG7

200053_at

++

12

0.031

GPAA1

201618_x_at

+

13

0.032

PSMD2

200830_at

+++

14

0.032

COX7A2L

201256_at

+++

15

0.033

CLTA

204050_s_at

+++

16

0.033

NDUFA2

209224_s_at

+++

17

0.033

SSR2

200652_at

++

18

0.034

TTC1

201434_at

++

19

0.034

RPLP1

200763_s_at

+++

20

0.034

INPP5K

202782_s_at

-

  1. Probe sets are listed according to rank. Higher rank indicates greater expression stability. Note that selection was narrowed to 544 probe sets likely to be most representative of genes of interest.
  2. "exp" = gene expression represented by hybridization signal intensity [52].
  3. "+" and "-" symbols represent signal intensity values averaged across all animals and obtained using Affymetrix® GCOS analysis, and global scaling to an average target intensity of 200. (-) = "undetectable", (+) = "signal intensity < 500", (++) = "signal intensity 500-1500", (+++) = "signal intensity 1501-10000". (++++) = "signal intensity > 10000".
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BMC Molecular Biology

ISSN: 1471-2199

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