Table 4 Primers used to amplify DHRS4L1 mRNA.
usage | Primer | Sequence (5'→3) |
|---|---|---|
5'RACE (touchdown PCR) | UPA-F | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT (long) CTAATACGACTCACTATAGGGC (short) |
| Â | DHRS4L1 E10-R | GAGCACAGGAAAGACACGATGCCAAGAG |
3'RACE (1st round of nest PCR) | DHRS4L1 outer F | GGATGGACAAGGAAAAAGAGG |
| Â | Adaptor 3'outer R | TACCGTCGTTCCACTAGTGATTT |
3'RACE (2nd round of nest PCR) | DHRS4L1 inner F | TTAGGCGAGCCAGAGGATTCTCTT |
| Â | Adaptor 3' inner R | CGCGGATCCTCCACTAGTGATTTCACTATAGG |
RT-PCR | DHRS4L1 Ea F | CAAGCCCACCGTGGAGCTCATCTGA |
| Â | DHRS4L1 E10-R | GAGCACAGGAAAGACACGATGCCAAGAG |
RT-PCR | DHRS4L1 E1 F | ATGCACAAGGCGCGGCTACGAG |
| Â | DHRS4L1 E10 R | GAGCACAGGAAAGACACGATGCCAAGAG |
RT-PCR | β-actin F | AAATCGTGCGTGACATTAA |
|  | β-actin R | CTCGTCATACTCCTGCTTG |