Figure 2

Transcription of DHRS4L1. Lane 1. BE(2)-M17, 2. SK-SY5Y, 3. SK-N-SH, 4. HeLa, 5. Eca-109, 6. Hep G2, 7. HL-7702, M. marker(100 bp DNA ladder). (A) β-actin shown as a control of RT-PCR. (B) RT-PCR amplifying the exon 1-exon 10 of DHRS4L1. Product of 493 bp is composed of exon 1-part of intron 2-exon 8-exon 9-exon 10. (C) RT-PCR amplifying the exon a 2-exon 10 of DHRS4L1. Product of 469 bp is composed of exon a2- exon 8-exon 9-exon 10. Product of 648 bp is composed of exon a2-part of insertion 3-exon 8-exon 9-exon 10.