Table 2 Average expression stability (M values) of the six potential reference genes according to geNorm.
Experiment | Organ | Treatment | ACTB1 | EF1A1 | HPRT | RPL7 | Tubb2C | UbcE |
|---|---|---|---|---|---|---|---|---|
Development | Larva | Dev1 | 0.365 | 0.363 (0.216) | 0.389 | 0.391 (0.279) | 0.434 | 0.360 (0.247) |
Dev2 | 0.885 | 0.529 (0.393) | 0.583 (0.435) | 0.483 (0.327) | 0.592 | 0.529 | ||
Adult | All | None | 1.464 | 0.782 (0.456) | 0.865 (0.557) | 0.774 (0.461) | 1.025 | 0.797 |
Infection | All | Mock | 2.008 | 0.969 (0.502) | 1.196 (0.656) | 1.011 (0.503) | 1.492 | 1.072 |
NNV | 1.985 | 0.953 (0.458) | 1.167 (0.640) | 0.972 (0.477) | 1.460 | 1.054 | ||
Brain | Mock | 0,620 | 0,363 | 0,330 (0,233) | 0,422 | 0,352 (0,248) | 0,314 (0,227) | |
NNV | 1,088 | 0,508 (0,332) | 0,500 (0,288) | 0,549 | 0,693 | 0,496 (0,291) | ||
Eye | Mock | 0,916 | 0,500 (0,354) | 0,483 (0,393) | 0,532 (0,362) | 0,606 | 0,490 | |
NNV | 0,864 | 0,451 (0,351) | 0,409 (0,258) | 0,462 | 0,527 | 0,414 (0,278) | ||
Thymus | Mock | 0,570 | 0,342 (0,271) | 0,321 (0,256) | 0,387 | 0,369 | 0,321 (0,254) | |
NNV | 0,791 | 0,429 (0,305) | 0,409 | 0,440 | 0,420 (0,270) | 0,394 (0,267) | ||
Spleen | Mock | 0,750 | 0,524 (0,400) | 0,577 (0,445) | 0,526 (0,388) | 0,567 | 0,514 | |
NNV | 1,359 | 0,632 (0,367) | 0,701 | 0,682 (0,410) | 0,988 | 0,656 (0,461) | ||
Ant. kidney | Mock | 0,750 | 0,478 (0,326) | 0,618 | 0,507 (0,386) | 0,564 | 0,455 (0,381) | |
NNV | 0,668 | 0,441 (0,314) | 0,569 | 0,442 (0,341) | 0,486 | 0,428 (0,370) | ||
Liver | Mock | 1,080 | 0,739 (0,429) | 0,793 (0,510) | 0,678 (0,407) | 1,091 | 0,664 | |
NNV | 0,968 | 0,623 (0,311) | 0,697 (0,408) | 0,601 (0,326) | 0,939 | 0,606 | ||
Leucocytes | A. kidney | All | 0.738 | 0.732 (0.321) | 0.719 | 0.627 (0.335) | 0.838 | 0.676 (0.304) |