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Table 2 Average expression stability (M values) of the six potential reference genes according to geNorm.

From: Evaluation of potential reference genes for real time RT-PCR studies in Atlantic halibut (Hippoglossus Hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes

Experiment Organ Treatment ACTB1 EF1A1 HPRT RPL7 Tubb2C UbcE
Development Larva Dev1 0.365 0.363 (0.216) 0.389 0.391 (0.279) 0.434 0.360 (0.247)
   Dev2 0.885 0.529 (0.393) 0.583 (0.435) 0.483 (0.327) 0.592 0.529
Adult All None 1.464 0.782 (0.456) 0.865 (0.557) 0.774 (0.461) 1.025 0.797
Infection All Mock 2.008 0.969 (0.502) 1.196 (0.656) 1.011 (0.503) 1.492 1.072
   NNV 1.985 0.953 (0.458) 1.167 (0.640) 0.972 (0.477) 1.460 1.054
  Brain Mock 0,620 0,363 0,330 (0,233) 0,422 0,352 (0,248) 0,314 (0,227)
   NNV 1,088 0,508 (0,332) 0,500 (0,288) 0,549 0,693 0,496 (0,291)
  Eye Mock 0,916 0,500 (0,354) 0,483 (0,393) 0,532 (0,362) 0,606 0,490
   NNV 0,864 0,451 (0,351) 0,409 (0,258) 0,462 0,527 0,414 (0,278)
  Thymus Mock 0,570 0,342 (0,271) 0,321 (0,256) 0,387 0,369 0,321 (0,254)
   NNV 0,791 0,429 (0,305) 0,409 0,440 0,420 (0,270) 0,394 (0,267)
  Spleen Mock 0,750 0,524 (0,400) 0,577 (0,445) 0,526 (0,388) 0,567 0,514
   NNV 1,359 0,632 (0,367) 0,701 0,682 (0,410) 0,988 0,656 (0,461)
  Ant. kidney Mock 0,750 0,478 (0,326) 0,618 0,507 (0,386) 0,564 0,455 (0,381)
   NNV 0,668 0,441 (0,314) 0,569 0,442 (0,341) 0,486 0,428 (0,370)
  Liver Mock 1,080 0,739 (0,429) 0,793 (0,510) 0,678 (0,407) 1,091 0,664
   NNV 0,968 0,623 (0,311) 0,697 (0,408) 0,601 (0,326) 0,939 0,606
Leucocytes A. kidney All 0.738 0.732 (0.321) 0.719 0.627 (0.335) 0.838 0.676 (0.304)
  1. Low M values indicate high expression stability, and recalculated M values are given in brackets. The recalculated M values are given for the three most stably expressed genes according to geNorm, after elimination of the three less stable genes.
  2. Abbreviations: Dev 1 - developmental stages before activation of zygotic transcription, Dev 2 - developmental stages after hatching, A. kidney - anterior kidney, Mock - mock-injected, NNV - NNV-injected.