Evaluation of potential reference genes for real time RT-PCR studies in Atlantic halibut (Hippoglossus Hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes

Table 1 Primers and probes used for real time RT-PCR analysis.

Gene	Forward	Revers	Probe	E%
ACTB1	CGGTCGTACCACAGGTATCGT	GGATCTTCATCAGGTAGTCAGTCAGA	TCTGGTGACGGT-MGB	90
EF1A1	CCATGGTTGTGGAGTCCTTCTC	GATGACACCGACAGCCACTGT	CTCCCCTCGGTCGTTTCGCTGTG	96
HPRT1	GTGGACTTCATTCGCCTCAAG	TCTCCACCGATAACTTTGATTTCA	ACTGGTCGTTACAGTAGC-MGB	98
RPL7	GAAGGCTCTCGGCAAATATGG	GCCAACTGTGTAAATCTCATGGAT	TCCTCGACGCAGATGA-MGB	94
Tubb2C	GCTGGACAGGATAAACGTGTATTTACA	GTGCCCGGCTCCAGATC	CCTCAGGCGGTAAAT-MGB	93
UbcE	GAACTGGAAACCAGCGACAAA	CGCTCACCAGAGCAATGAGA	ACCATCCAAGTGATCAATA-MGB	98

The primers and probes are listed 5' → 3' direction with the corresponding PCR efficiency (E%). All probes are marked with 6-carboxyfluorescein (6FAM). Abbreviation: MGB - minor groove binder.

ISSN: 1471-2199