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Table 1 Candidate qPCR reference genes tested in this study with the putative function of the proteins they encode and accession numbers of the produced epaulette shark gene sequences.

From: Elasmobranch qPCR reference genes: a case study of hypoxia preconditioned epaulette sharks

Symbol

Gene (homolog) name

Function

Genbank accession

actc1

alpha-actin, cardiac muscle 1

Structural protein

GU983379

dnaja2

dnaJ subfamily A 2 (Hsp40)

Chaperone

GQ152302

eef1b

eukaryotic translation elongation factor 1 beta

Protein synthesis

GU983380

hprt

hypoxanthine phosphoribosyltransferase 1

Purine nucleotide synthesis

GQ152303

mrip

myosin phosphatase-Rho interacting protein

Component of the myosin phosphatase complex

GQ152304

polr2f

polymerase (RNA) II (DNA directed) polypeptide F

Component of RNA polymerase II

GQ152305

rpl6

60S ribosomal protein L6

Component of the large 60S ribosomal subunit

GU983381

tubb2

tubulin beta 2

Structural protein

*

ubq

ubiquitin

Protein degradation

GQ152306

  1. * qPCR primer designed directly from the tubb2 alignment of following vertebrate species: human ENST00000340384, chicken ENSGALT00000022476, frog ENSXETT00000047287, stickleback ENSGACT00000016810, zebrafish BC062827.1