Figure 3

Kunitz-type SPIs of B. flaviceps venom gland cDNA library. Similar sequences were clustered together and a representative of the each cluster is presented. B. flaviceps Kunitz-type SPIs can be divided into three distinct groups (A, B and C). The deduced amino acid sequences of isoforms of each group are shown. The amino residues which are different from the representative of major isoform are shaded. The predicted signal peptide of the transcripts is shown in bold. The major isoforms from each group is aligned with closely related protein found in the database and the % identity and similarly are also shown in the figure. A) Putative clusters of β-bungarotoxin B chains of B. flaviceps and alignment with IVB_BUNFL (Q7T2Q6.1). The extra cysteine residue that is involved in the disulphide bridge with chain A is underlined. B) Putative Kunitz-type SPIs of B. flaviceps and alignment with IVBI5_BUNMU (Q1RPS9.1) chain B5 precursor of Bungarus multicinctus, IVB_BUNFL (Q7T2Q6.1) B. flaviceps, Dendrotoxin-K (P00981.2) and Dendrotoxin-I (P00979) from Dendroaspis polylepis polylepis. C) Putative Kunitz-type SPI and its truncated form. Alignment of the major isoform with Kunitz inhibitor IV (ABX82870.1) from W. aegyptia is also shown.