Figure 2
From: Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
Regulation of activation of 136 bp length ZC3H12A promoter fragment. Luciferase activity was measured to examine the regulation of activation of 136 bp length ZC3H12A promoter fragment. HepG2 cells transiently transfected with the luciferase construct containing the fragment of human ZC3H12A promoter located between -76 bp and +60 bp. Increasing amounts of pElk-En (20, 50, 100, 200 ng - lanes 2, 3, 4, 5) or Elk-VP16 (20, 50, 100, 200 ng - lanes 6, 7, 8, 9) were co-transfected to the cells. Lane 1 - control cells without Elk-En or Elk-VP16. Luciferase activity was measured 24 h after transfection. Statistical significance was determined using the Student's test. *P < 0.05, **P < 0.001.