Figure 4

Treatment of hOBs with the glycogen synthase kinase (GSK-3β) inhibitor, SB216763. (A) A scheme of SB216763 action mechanism is reported (see the text for details). (B) Effect of SB216763 on the TOPflash reporter system. 24 h after transient transfection with the TOPflash plasmid, the cells were treated (+) or not (-) with SB216763 (10 μM) for 24 h prior to harvest. Luciferase activity was normalized to β-galactosidase activity in the same sample. The bars represent mean ± SEM. * = p < 0.05. (C) The levels of β-catenin expression, SLUG and RUNX2 were examined by Western blot in hOBs treated with SB216763 (10 μM) or with the only vehicle (-). The quantitative presentation of the protein levels was performed by densitometric analysis using Anti-IP(3)K as control. D.U. = densitometric units. A representative Western blot analysis with size markers (KDa) is reported. * = p < 0.05.