Table 4 Candidate endogenous control (EC) genes and their PCR amplification efficiencies (E)
EC | Function | Chromosomal Location | Amplicon Size (bp) | Assay Identifier* | E (%) |
|---|---|---|---|---|---|
B2M | Defence/immunity | 15q21-22.2 | 64 | Hs00187842_m1 | 101.8 |
GAPDH | Oxidoreductase, dehydrogenase | 12p13 | 122 | Hs99999905_m1 | 99.8 |
PPIA | Isomerase | 7p13 | 98 | Hs99999904_m1 | 96.6 |
HPRT | Glycosyl transferase | Xq26.1 | 100 | Hs99999909_m1 | 97.9 |
MRPL19 | Protein biosynthesis | 2q11.1-11.2 | 72 | Hs00608519_m1 | 102.2 |
ACTB | Cytoskeletal structure | 7p15-12 | 171 | Hs99999903_m1 | 95.2 |
DTX3 | Signals transduction | 12q13.3 | 64 | Hs00400987_m1 | 99.1 |
SLC25A23 | Mitochondrial carrier | 19p13.3 | 86 | Hs00225469_m1 | 97.8 |
CHRNB4 | Nicotinic receptor | 15q24 | 75 | Hs00609523_m1 | 103.6 |
RTDR1 | Aminopeptidase transport | 22q11.2 | 112 | Hs00205353_m1 | UD |
HCRT | Homeostatic regulator | 12q21 | 101 | Hs00533664_m1 | UD |
APOC4 | Apo-lipoprotein | 19q.2 | 144 | Hs00155791_m1 | UD |
KRTAP12-3 | Acetylgalactoa-minyltransferase | 3q25 | 83 | Hs01651247_s1 | UD |