Identification of endogenous control genes for normalisation of real-time quantitative PCR data in colorectal cancer

Table 2 Cycle threshold (Ct) values of candidate EC genes and PCDC4 in colorectal tissues

EC Gene	C_t Range	C_t Min	C_t Max	Mean C_t ± s.e.m	Standard deviation (SD)
B2M	4.03	17.47	21.51	19.48 ± 0.14	1.04
PPIA	4.13	17.78	21.91	19.90 ± 0.14	1.06
GAPDH	5.80	18.51	24.32	21.00 ± 0.17	1.29
ACTB	12.74	21.08	33.32	25.14 ± 0.34	2.61
HPRT	8.54	22.74	31.28	26.68 ± 0.25	1.89
DTX3	6.6	24.95	31.56	28.62 ±0.17	1.37
SLC25A23	7.26	24.48	31.74	27.36 ±0.19	1.54
CHRNB4	9.40	27.99	37.38	32.30 ±0.19	2.15
RTDR1	-	30.59	UD	35.82 ±0.36	2.15
HCRT	-	33.96	UD	38.46 ±0.29	1.67
APOC4	-	UD	UD	-	-
KRTAP12-3	-	33.16	UD	36.95 ±0.19	1.46
MRPL19	4.10	26.70	30.80	28.62 ± 0.13	0.98
CXCL12	13.54	21.85	35.39	25.77 ± 0.32	2.61
FABP1	15.57	16.61	32.19	20.83 ± 0.40	3.24
MUC2	17.71	17.62	35.33	22.43 ± 0.53	4.16
PDCD4	11.92	21.35	33.27	24.56 ± 0.32	2.59

B2M and PPIA were the most abundantly expressed genes, having the lowest mean Ct values while MRPL19 was the least abundantly expressed gene with average Ct values > 26. Both B2M and PPIA had the lowest range in their Ct values.

ISSN: 1471-2199