Inflammation in the avian spleen: timing is everything

BMC Molecular Biology

Table 1 List of primers used for qRTPCR.

Target	Identity	Sequence	Accession #	Size
Bmal1	Fwd	ggaattccaggaggaacaaga	AF205219	60
	Rv	ttcttcagcaatcatccttcc
Bmal2*	Fwd	gaagtccggtataaaccttcgtt	AF246958	65
	Rv	gcagccctaaggattaactgtct
Clock	Fwd	acacgcatgatagaggcaaa	AF246959	62
	Rv	tgttcttgaattttccgcaact
Cry1*	Fwd	cggacctgtacaaaaaggtaaaa	NM_204245	61
	Rv	agctggccatagagggagag
Cry2	Fwd	tctggcgggagtttttctac	NM_204244	60
	Rv	cctccatgcgatcaaacttc
Per2	Fwd	cgaggtcagggggttctact	NM_204262	61
	Rv	gatatcagcttgctgctcagg
Per3*	Fwd	tttaggctctcactcctgtgaa	AY046567	60
	Rv	ttgctgtttttcccactgtct
IL-1β	Fwd	ggtggccatgaccaaact	NM_204524	61
	Rv	caggtcgctgtcagcaaag
IL-2	Fwd	gagtgcacccagcaaactct	NM_204153	66
	Rv	ttcagtttctttcttcagagtaacca
IL-6*	Fwd	caggacgagatgtgcaagaa	NM_204628	64
	Rv	tgttccggacgagcatct
IL-12b	Fwd	ccaccgaagtgaaggagttc	NM_213571	63
	Rv	cgtgggtcttagcagacagg
IL-18*	Fwd	agagcatgggaaaatggttg	NM_204608	60
	Rv	ccaggaatgtctttgggaac
TNF ά	Fwd	acaaaatttgcaggctgtttc	AY765397	60
	Rv	ctgaaataaacaggcacaaaagag
GAPDH*	Fwd	ggagtccactggtgtcttcac	NM_204305	64
	Rv	cttagcaccacccttcagatg

Fwd = forward primer; Rv = reverse primer; Size = expected amplicon size. Primer pairs that also spanned an intron are indicated by an *.

ISSN: 1471-2199