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Table 3 Expression analysis of target genes.

From: qPCR in gastrointestinal stromal tumors: Evaluation of reference genes and expression analysis of KIT and the alternative receptor tyrosine kinases FLT3, CSF1-R, PDGFRB, MET and AXL

 

Gene

Factor

p-value

 

Gene

Factor

p-value

NT↔WT

KIT

5.9+

0.095

NT↔NF1

KIT

17+

0.003

 

CSF1R

3.99-

0.217

 

CSF1R

2.99-

0.294

 

FLT3

2.43-

0.498

 

FLT3

3.985-

0.179

 

PDGFRB

5.79-

0.087

 

PDGFRB

2.827-

0.196

 

AXL

2.1-

0.66

 

AXL

2.5+

0.53

 

MET

8.7-

0.034

 

MET

6.5-

0.13

NT↔Ex11

   

NT↔Ex18

   
 

KIT

11.7+

0.011

 

KIT

1.66+

0.373

 

CSF1R

4.65-

0.155

 

CSF1R

4.7-

0.051

 

FLT3

2.383-

0.41

 

FLT3

2.113-

0.263

 

PDGFRB

6.3-

0.046

 

PDGFRB

4.925-

0.007

 

AXL

3-

0.27

 

AXL

1.35-

0.58

 

MET

17.81-

0.001

 

MET

7.7-

0.001

NT↔Ex9

   

Ex11↔WT

   
 

KIT

5.261+

0.05

 

KIT

1.981-

0.232

 

CSF1R

13.98-

0.003

 

CSF1R

1.164+

0.861

 

FLT3

5.38-

0.022

 

FLT3

1.023-

0.971

 

PDGFRB

12.78-

0.001

 

PDGFRB

1.087+

0.920

 

AXL

3.4-

0.08

 

AXL

1.4+

0.6

 

MET

9.766-

0.001

 

MET

2+

0.33

  1. Expression analysis of KIT, CSF1R, FLT3, PDGFRB, AXL and MET was carried out in six GIST cohorts (NT = normal tissue, WT = wild type, Exon 9, Exon 11, NF1 = neurofibromatosis type 1, Exon 18). Significant variances are in bold. The expression data were calculated using the REST software with POLR2A as reference gene. NT: normal tissue; p < 0.05; +/- indicates up- or downregulation