Figure 3

TAF6δ controls protein expression and promoter-dependent transcription. (A) HeLa cells were treated with control (ctrol), TAF6δ-inducing (SSO T6-3), or Bcl-xS-inducing (SSO Bcl-x) splice-switching oligonucleotides. Total protein extracts were prepared 18 hours post-transfection and fractionated by SDS-PAGE followed by immunoblot analysis of endogenous protein levels with antibodies indicated at the left. (B) Promoter-dependent regulation of transcription by endogenous TAF6δ. HeLa cells were transfected with 100 to 200 ng of HES1, DUSP1, BAX or ADM promoter-luciferase constructs. 24 hours post-transfection the cells were treated with 100 nM TAF6δ-inducing oligonucleotide for another 24 hours before measurement of luciferase activity (y-axis). Results are expressed as log₂ fold change of TAF6δ SSO versus control SSO treated cells. Error bars indicate standard deviation of three independent transfections.