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Figure 9 | BMC Molecular Biology

Figure 9

From: Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

Figure 9

Degree of correlation between normalization strategies in more heterogeneous datasets. Scatter plot of ratio values obtained from sample set "K" for genes which together illustrated a wide range of responses (from strong over-expression to repression, see Figure 8 for some of them). X axis: ratios calculated using the three most stable genes (NF(UBC 6, TFC 1, KRE 11)); Y axis: ratios calculated using NF(TAF 10, FRP 2, ALG 9)(A) and NF(ACT 1)(B). Dotted line: y = x. The equation and correlation coefficient of the linear regression fit (not reported) were y = 1.030x, R2 = 0.916 (A) and y = 2.405x, R2 = 0.124 (B).

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