Figure 3
From: RAD51 paralogs promote homology-directed repair at diversifying immunoglobulin V regions
RAD51, RAD51D-GFP and XRCC2-GFP foci localize to the diversifying λ R gene in DT40 PolyLacO-λ R cells. (A) Igλ locus tagged with polymerized lactose operator (PolyLacO) in DT40 PolyLacO-λR. The ψVλ array, PolyLacO, VJ and Cλ regions are indicated. (B) Immunofluorescent images of colocalizations of λR gene with RAD51 in single DT40 PolyLacO-λR RFP-LacI cells. Images of two representative cells are shown. λR, red signal from RFP-LacI binding at IgλR; α-RAD51, green; merge, λR and RAD51 signal; DAPI, nuclear DNA. Bar, 10 μm. (C) Immunofluorescent images of colocalizations of the tagged λR gene with RAD51D-GFP in stable DT40 PolyLacO-λR RFP-LacI RAD51D-GFP transfectants. λR, red signal from RFP-LacI binding at IgλR; α-GFP, green; merge, λR and GFP signal; DAPI, nuclear DNA. Bar, 10 μm. (D) Immunofluorescent images of colocalizations of the tagged λR gene with XRCC2-GFP in stable DT40 PolyLacO-λR RFP-LacI XRCC2-GFP transfectants. Notations as in panel C.