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Figure 3 | BMC Molecular Biology

Figure 3

From: Dual effect of a single nucleotide polymorphism in the first intron of the porcine Secreted phosphoprotein 1 gene: allele-specific binding of C/EBP beta and activation of aberrant splicing

Figure 3

Analysis of binding affinity of the probes allelic for the SNP g.3836A>G by competitive EMSA using nuclear extract from cells overexpressing N-terminally truncated bovine C/EBPβ. (A) Competitive EMSA was performed using labelled probe A and 5-100 fold molar excess of cold competitor probes A (lanes 2-6) and G (lanes 7-11) respectively. In the first lane EMSA was performed without competitor. (B) The relative band intensity (EMSA without competitor representing the basal level of 100%) plotted vs. increasing concentrations of allelic competitor probes.

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