Figure 5
From: Quadruple 9-mer-based protein binding microarray with DsRed fusion protein
EMSA-based competition analysis of OsNAC6. Candidate duplexes containing 'A(A/C)GTAA' and G-rich sequences were used as core binding sequences for the interaction. The mobility shifts were performed using 5 μg of enriched OsNAC6 protein and 40 fmol of biotinylated oligonucleotides. The shift was assessed by competition using unlabeled oligonucleotides. The molar excess of competitor used was over 1,000 fold. Lane 1; OsNAC6 + biotin-labeled DNA. Lane 2; OsNAC6 + biotin-labeled DNA + unlabeled DNA (40 pmol). Lane 3; OsNAC6 + biotin-labeled DNA + unlabeled DNA (80 pmol). Lane 4; OsNAC6 + biotin-labeled DNA + unlabeled DNA (120 pmol). Lane 5; OsNAC6 + biotin-labeled DNA. Lane 6; OsNAC6 + biotin-labeled DNA + unlabeled DNA (40 pmol). Lane 7; OsNAC6 + biotin-labeled DNA + unlabeled DNA (60 pmol). Lane 8; OsNAC6 + biotin-labeled DNA + unlabeled DNA (120 pmol).