Figure 2

Scan images of protein binding microarray. DsRed-fused Cbf1 PBM image was compared to that of DsRed-monomer. DsRed was displayed in green color in contrast with red probes. Cy5 intensity depends on the base composition of probes because Cy5 fluorescent dUTP was incorporated during the synthesis of complimentary DNA strands. The arrows indicate corresponding quadrupled 9-mer sequences of probes in each microarray. 1, GTCACGTGA; 2, ACACGTGTG; 3, CGTGGGCCA; 4, ACACCCGTG; 5, GATCACGGG. (A) DsRed-fused Cbf1 was efficiently bound to the double-stranded probes. Because 'CACGTG' sequence is a Cbf1 binding motif [16], any quadrupled 9-mer probe including 'CACGTG' showed higher DsRed fluorescent intensity than other variant probes. (B) There was no specific binding of DsRed without fusion of DNA binding protein.