Table 1 Primers used in BAC library screen, promoter analysis and real-time RT-PCRs
From: Promoter analysis of the rabbit POU5F1 gene and its expression in preimplantation stage embryos
Primer name | Primer (5'-3') sequence | Position | Primer size (bp) | Tm (°C) |
|---|---|---|---|---|
oct4-435-F | AGCTTAGCTTCAAGAACATG | +3665 | 20 | 60.0 |
oct4-435-R | AGGAGTACAGTGCAGTGAAG | +4704 | 20 | 60.0 |
oct4-186-F | AGCAGAAACCCTCGTGCAGG | +3759 | 20 | 60.0 |
oct4-186-R | TCTGGCGCCGGTTACAGAAC | +4181 | 20 | 60.0 |
*oct4-pseudo-F | GCTAAACAGAAAGAAGTTTGCC | * +420 | 22 | 57.0 |
*oct4-pseudo-R | GAACAGTCACTGCTTGATCGTTT | * +870 | 23 | 58.1 |
oct4-cDNA-F | GCTCTACAGAAAGAACTCGAGCAG | +3640 | 24 | 57.9 |
oct4-cDNA-R | CGAGTACAGGGTAGCAAAGTGAG | +4869 | 23 | 60.0 |
CR1-ATGmut | TCTATGGGGGAAGGAGGGCG | +5 | 20 | 60.0 |
CR1 | AGGCTGGTGGCATAAAACAC | -558 | 20 | 60.0 |
CR1+(CR2+CR3) | GCCAGACTAGAGCCCAACAG | -1585 | 20 | 60.0 |
CR1+(CR2+CR3)+CR4 | GGGAAATTGTGGAGGAGGAC | -2030 | 20 | 60.7 |
CR4 | TCTGTCTGCTTGGTGGTGTC | -1670 | 20 | 59.9 |
Rabbit-oct4-RT-F | CGAGTGAGAGGCAACTTGG | +4418 | 19 | 55.5 |
Rabbit-oct4-RT-R | CGGTTACAGAACCACACACG | +4730 | 20 | 56.1 |
*Rabbit-oct4p-RT-F | CTAAACAGAAAGAAGTTTGCC | * +421 | 21 | 53.7 |
*Rabbit-oct4p-RT-R | CGCAGCTTACACATGTACT | * +594 | 19 | 52.3 |
Mouse-oct4-RT-F | ATGCCGTGAAGTTGGAGAAG | +343 | 20 | 56.5 |
Mouse-oct4-RT-R | GGTCTGGCTGAACACCTTTC | +3309 | 20 | 55.9 |