Table 2 KD Values as determined by EMSA and anisotropy
Conditions | KD (μM) | BMax | R value | |
|---|---|---|---|---|
EMSA | ||||
Wild type Ku | Control | 29.7 ± 2.5 | -- | 0.99 |
Ku70/80ΔC | Control | 51.0 ± 7.7 | -- | 0.99 |
Anisotropy | ||||
Wild type Ku | Control | 25.8 ± 2.3 | 0.24 | 0.995 |
Oxidized | -- | -- | -- | |
Re-reduced | 31.4 ± 3.0 | 0.20 | 0.98 | |
Ku70/80ΔC | Control | 25.7 ± 1.5 | 0.255 | 0.999 |
Oxidized | -- | -- | -- | |
Re-reduced | <50 | -- | -- |