Figure 4

Overexpression of NF-κB activates Neph3 promoter in A293 cells. The expression vector for p50 or/and p65 NF-κB (NF-κB family members p50 and p65) was co-transfected with the Neph3 5' -105-Luc reporter plasmid or with reporter plasmid containing a mutated NF-κB binding site (Neph3 5' -105/NF-κBmut -Luc) into A293 cells and luciferase activities were measured 24 h after transfection. The activities produced by the studied constructs were normalized against co-transfected control plasmid (phRL-TK) activities. The results are presented as fold induction of the control experiment co-transfected with corresponding empty vector. The values represent means +S.D. from three independent experiments performed in triplicates. Statistical difference of the promoter activity, using independent samples t-test, is shown against the control co-transfection. *** p < 0.001.