Figure 1
From: Selection of reference genes for expression studies with fish myogenic cell cultures
Growth and differentiation of myogenic cells extracted from Salmo salar fast myotomal muscle. Growth is shown at 2 d (a-d), 5 d (e-h), 8 d (i-l), 11 d (m-p) and 14 d (q-t) after cell extraction. Myogenic cells were identified by positive desmin staining (a,e,i,m,q). Actin counterstained with phalloidin (b,f,j,n,r) and nuclei stained with sytox green (c,g,k,o,s) also confirmed the presence of multinucleated myotubes shown in the overlay (d,h,l,p,t). Scale bars represent 50 μm.