Table 2 Oligonucleotide primers used in this study
Primers | Sequence (5'-3') |
|---|---|
| Â | (A) Primers for RT-PCR expression profiling |
Ex1a-B |    CAGGGAGACAGCTGATACATTTAG |
Ex1a-A |    TCCAGCCAGAGAAAGACTCATCATC |
T1SP-F1 |    CCCACTTCTTCAGAATTCCAGCAT |
T1SP-R1 |    GTTCAAGACATCAGACAAGAATAA |
T1SP-R2 |    CTTTAGTCTTCAGGTTCTCTGCTC |
T1SP-R3 |    AACATCTGAGATCTTTGTAATAAC |
T2SP-F1 |    CGCACATCTTCAGGATTCTGGTCG |
T2SP-R1 |    AGACATCAAACAAGAATCCAACAG |
T2SP-R2 |    CCCTAGTCTTCAGGTGCTCTGTTT |
T2SP-R3 |    AACATTTGACATTTTTGTAATAAG |
2965R |    GAGATTGCCACACAGATGATAGAG |
3096R |    TTGACCATATAAACCAACTGACAG |
| Â | (B) 5'- and 3'-RACE gene-specific primers |
5' RACE-GSP-R1 |    CACCAGGCTGCAGTCTGTGAAGAGG |
5' RACE-GSP-R2 |    CTTCACTGGCCTCTAATGAGAACC |
3' RACE-GSP-F1 |    AGATTACCTGTCTGTTTACCTGGG |
3' RACE-GSP-F2 |    TTGGGCAAAGGTACAGTTCTGGAT |
| Â | (C) Primers for cloning of 5'-UTR for uORF assays |
T1 uORF-F |    TTGCGTCCGGCTCTCAGC |
T1 uORF-R NcoI |    AACCATGG TTTCTGGAGGTAGTTTGGA |
| Â | (D) Primers for site-directed mutagenesis |
F215 |    CCTGAAGAGTAAGCAGACCAAGAGAACATAGAG |
R247 |    CTCTATGTTCTCTTGGTCTGCTTACTCTTCAGG |
F373 |    GTCTATATAAGCTCAAGGAAGAAGGAAGC |
R401 |    GCTTCCTTCTTCCTTGAGCTTATATAGAC |
| Â | (E) Primers for luciferase RT-PCR |
Luc-F (1265–1288) |    ACATAGCTTACTGGACGAAGACG |
Luc-R (1557–1580) |    GTAAGACCTTTCGGTACTTCGTCC |