Table 2 Primer sequences and amplicon characteristics for each of the 11 reference genes
From: Selection of reliable reference genes for gene expression studies in peach using real-time PCR
Name | Forward Primer Sequence [5'-3'] | Reverse Primer Sequence [5'-3'] | Amplicon Size (bp) | Product TM (°C) # | RT-qPCR Efficiency* | R2* |
|---|---|---|---|---|---|---|
18S rRNA | TAGTTGGTGGAGCGATTTGTCTG | CTAAGCGGCATAGTCCCTCTAAG | 114 | 88.2 | 1.796 | 0.9995 |
ACT | GTTATTCTTCATCGGCGTCTTCG | CTTCACCATTCCAGTTCCATTGTC | 112 | 86.3 | 1.828 | 0.9993 |
CYP2 | ACTCCAAAGCGTGTTAGAAAAGG | GTCTCTTCCACCATAACGATAGG | 120 | 90.4 | 1.767 | 0.9986 |
TEF2 | GGTGTGACGATGAAGAGTGATG | TGAAGGAGAGGGAAGGTGAAAG | 129 | 88.3 | 1.818 | 0.9994 |
GAPDH | ATTTGGAATCGTTGAGGGTCTTATG | AATGATGTTGAAGGAAGCAGCAC | 121 | 88.7 | 1.794 | 0.9994 |
PLA2 | TCGCCGTCGTTATCTTCTCC | TACCGAATCCCAACAGAATTACAG | 115 | 90.8 | 1.765 | 0.9995 |
RP II | TGAAGCATACACCTATGATGATGAAG | CTTTGACAGCACCAGTAGATTCC | 128 | 85.3 | 1.800 | 0.9996 |
RPL13 | GCAGCGACTGAAGACATACAAG | GGTGGCATTAGCAAGTTCCTC | 103 | 87.7 | 1.671 | 0.9952 |
TUA | TTCTCTCTACTCATTCCCTCCTTG | GATTGGTGTATGTTGGTCTCTCG | 117 | 83.9 | 1.812 | 0.9993 |
TUB | CCGAGAATTGTGACTGCCTTCAAG | AGCATCATCCTGTCTGGGTATTCC | 124 | 88.2 | 1.826 | 0.9994 |
UBQ10 | AAGGCTAAGATCCAAGACAAAGAG | CCACGAAGACGAAGCACTAAG | 146 | 89.5 | 1.795 | 0.9994 |