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Figure 1 | BMC Molecular Biology

Figure 1

From: Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the Cephalopod Mollusc Octopus vulgaris

Figure 1

RT-qPCR cycle threshold (Ct) values of candidate reference genes among different tissues in two data sets. Cycle threshold distribution values (Ct) of 16S, actB, tubA, EEF1A and ubi (set a, left panel) and 18S, actB, tubA, EEF1A, ubi (set b, right panel) from mantle, supraoesophageal and suboesophageal masses and optic lobes of O. vulgaris. The distribution is shown by vertical box plot as medians (lines), interquartile range (boxes) and ranges (whiskers). Circles mark outliers with values between 1.5 and 3 times the interquartile range; asterisks mark outliers having Ct more than three times the interquartile.

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