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Figure 9 | BMC Molecular Biology

Figure 9

From: Cooperation between NRF-2 and YY-1 transcription factors is essential for triggering the expression of the PREPL-C2ORF34 bidirectional gene pair

Figure 9

Semi-quantitative PCR products from DNA prepared by ChIP assays. Lanes 1 and 5: No DNA control. Lanes 2 and 6: The Input lanes correspond to PCR products derived from a 1/10 dilution of chromatin prior to immunoprecipitation. Lanes 3 and 7: The IgG lanes correspond to PCRs containing chromatin immunoprecipitated with antibodies against control IgG. Lanes 4 and 8: The NRF-2α and YY-1 lanes correspond to PCRs containing chromatin immunoprecipitated with antibodies against NRF-2α and YY-1, respectively. All the amplified products represent a 254-bp region (from position -66 to -319) containing both the NRF-2 and YY-1 binding sites of the PREPL-C2ORF34 bidirectional gene pair. Additional ChIP assays were performed in other cell lines with similar results (presented in Additional File 4).

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BMC Molecular Biology

ISSN: 1471-2199

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