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Table 4 Expression Stability of GOI Evaluated by BestKeeper Software

From: Identification of valid reference genes during the differentiation of human myoblasts

 

ACTA1

MYOG

MYH3

n

10.00

10.00

10.00

GM [CP]

21.95

24.56

30.07

AM [CP]

22.21

24.78

30.21

min [CP]

16.92

20.10

27.05

max [CP]

27.14

30.06

35.25

SD [± CP]

3.06

2.88

2.75

CV [% CP]

13.77

11.62

9.11

min [x-fold]

-33.99

-21.40

-8.49

max [x-fold]

38.16

43.48

39.50

SD [± x-fold]

8.55

7.21

7.04

  1. Descriptive statistics of three GOI based on their crossing point (CP) values. Abbreviations: AM [CP]: the arithmetic mean of CP; CV [%CP]: the coefficient of variance expressed as a percentage on the CP level; GM [CP]: the geometric mean of CP; min [CP] and max [CP]: the extreme values of CP; Min [x-fold] and Max [x-fold]: the extreme values of expression levels expressed as an absolute x-fold over- or under-regulation coefficient; n: number of samples; SD [± CP]: the standard deviation of the CP; SD [± x-fold]: standard deviation of the absolute regulation coefficients. Results from three independent experiments are shown.