Figure 4

Presence of nucleolin like variant (NRP) in spermatogenic cells. A) Immunolocalisation of nucleolin using goat polyclonal anti-nucleolin followed by alexa dye conjugated anti goat in round spermatids. Haploid cells, (panel a-b); and meiotic spermatocytes, (panel c-d). White arrows represent the round spermatid cells. B) Western blot analysis of nucleolin using rabbit polyclonal anti-nucleolin antibodies. 2n, diploid germ cells; 4n, meiotic spermatocytes and n spermatid cells. 2n diploid cells show two bands in contrast to the one band shown in 4n meiotic spermatocytes and n spermatid cells. C) Semiquantitative RT-PCR of full-length nucleolin mRNA. Lane 1 – marker; lane 2 – 2n, diploid germ cell; lane 3 – 4n, meiotic spermatocytes; and lane 4 – n, spermatid cells.